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primary mouse anti troponin t antibody  (Developmental Studies Hybridoma Bank)


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    Developmental Studies Hybridoma Bank primary mouse anti troponin t antibody
    Primary Mouse Anti Troponin T Antibody, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 269 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+mouse+anti+troponin+t+antibody/anti-Troponin+T/pmc12946758-533-3-9
    Average 96 stars, based on 269 article reviews
    primary mouse anti troponin t antibody - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Staining:

    Article Title: African pygmy mouse iPSCs as a model for in vitro embryogenesis, interspecies chimerism, and blastocyst complementation.
    Article Snippet: .. To stain cardiomyocytes, primary mouse anti-Troponin T antibody (2μg/ml, DSHB, CT3) in PBS containing 2% BSA was added overnight at 4 ◦ C. The next day, cells were washed 2x with PBS, followed by incubation with secondary anti-mouse IgG2a Alexa Fluor 546 antibody (1:400, Thermo Fisher Scientific, A-21133) and DAPI (1:1000) for 1h at RT. .. To stain live cultures of APM-iPSCs, FITC anti-mouse/human CD15 (SSEA1) antibody (1:20, BioLegend, 125612) was added directly to the culture medium of APM-iPSCs.

    Incubation:

    Article Title: African pygmy mouse iPSCs as a model for in vitro embryogenesis, interspecies chimerism, and blastocyst complementation.
    Article Snippet: .. To stain cardiomyocytes, primary mouse anti-Troponin T antibody (2μg/ml, DSHB, CT3) in PBS containing 2% BSA was added overnight at 4 ◦ C. The next day, cells were washed 2x with PBS, followed by incubation with secondary anti-mouse IgG2a Alexa Fluor 546 antibody (1:400, Thermo Fisher Scientific, A-21133) and DAPI (1:1000) for 1h at RT. .. To stain live cultures of APM-iPSCs, FITC anti-mouse/human CD15 (SSEA1) antibody (1:20, BioLegend, 125612) was added directly to the culture medium of APM-iPSCs.

    other:

    Article Title: African pygmy mouse iPSCs as a model for in vitro embryogenesis, interspecies chimerism, and blastocyst complementation
    Article Snippet: Next, cells were washed 2x with PBS and blocked/permeabilized with 2% bovine BSA (AppliChem, A1391) and 1% Triton X-100 (Sigma-Aldrich, X100-100ML) in PBS for 1h at RT.



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    Figure 6. Comparison of gene expression profiles associated with cardiomyocyte differentiation on day 8 between a positive control, negative control, and M5 when used during the Wnt inhibition step. NKX2-5, <t>TNNT2,</t> and MYH7 are cardiomyocyte marker genes. The vertical axis measures gene expression relative to the negative control. The error bars correspond to the means ± standard deviation (sample size = 4). Asterisks indicate significance as determined from one-way ANOVA test comparing to the negative control (****p < 0.001, ***p < 0.01, **p < 0.1).
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    Image Search Results


    Figure 6. Comparison of gene expression profiles associated with cardiomyocyte differentiation on day 8 between a positive control, negative control, and M5 when used during the Wnt inhibition step. NKX2-5, TNNT2, and MYH7 are cardiomyocyte marker genes. The vertical axis measures gene expression relative to the negative control. The error bars correspond to the means ± standard deviation (sample size = 4). Asterisks indicate significance as determined from one-way ANOVA test comparing to the negative control (****p < 0.001, ***p < 0.01, **p < 0.1).

    Journal: Journal of chemical information and modeling

    Article Title: Molecular Design for Cardiac Cell Differentiation Using a Small Data Set and Decorated Shape Features.

    doi: 10.1021/acs.jcim.4c01353

    Figure Lengend Snippet: Figure 6. Comparison of gene expression profiles associated with cardiomyocyte differentiation on day 8 between a positive control, negative control, and M5 when used during the Wnt inhibition step. NKX2-5, TNNT2, and MYH7 are cardiomyocyte marker genes. The vertical axis measures gene expression relative to the negative control. The error bars correspond to the means ± standard deviation (sample size = 4). Asterisks indicate significance as determined from one-way ANOVA test comparing to the negative control (****p < 0.001, ***p < 0.01, **p < 0.1).

    Article Snippet: To investigate cardiac differentiation, the substrates were incubated with mouse monoclonal cTnT primary antibody (MAB 1874, R&D Systems) and rabbit monoclonal MYH7 primary antibody (MAB 1874, R&D Systems) and incubated with goat antimouse and antirabbit secondary antibodies, under the conditions described in Supporting Information 6.

    Techniques: Comparison, Gene Expression, Positive Control, Negative Control, Inhibition, Marker, Standard Deviation

    Figure 7. Immunostaining analysis of cTnT of human iPS cell-derived cardiomyocytes differentiated for 8 days using the M5 molecule at 2 μM concentration for the Wnt inhibition step. Images were obtained by optical microscopy. Bars correspond to 30 μm.

    Journal: Journal of chemical information and modeling

    Article Title: Molecular Design for Cardiac Cell Differentiation Using a Small Data Set and Decorated Shape Features.

    doi: 10.1021/acs.jcim.4c01353

    Figure Lengend Snippet: Figure 7. Immunostaining analysis of cTnT of human iPS cell-derived cardiomyocytes differentiated for 8 days using the M5 molecule at 2 μM concentration for the Wnt inhibition step. Images were obtained by optical microscopy. Bars correspond to 30 μm.

    Article Snippet: To investigate cardiac differentiation, the substrates were incubated with mouse monoclonal cTnT primary antibody (MAB 1874, R&D Systems) and rabbit monoclonal MYH7 primary antibody (MAB 1874, R&D Systems) and incubated with goat antimouse and antirabbit secondary antibodies, under the conditions described in Supporting Information 6.

    Techniques: Immunostaining, Derivative Assay, Concentration Assay, Inhibition, Microscopy